For the last four years, with partners, IS-Instruments Ltd has had the ambition to produce a compact easy to use Deep UV resonant Raman spectrometer with the power to observe organic substances such as protein and enzyme, while providing a fluorescent-free measurement for all samples. Today, we can announce our first observations from ODIN, our Deep UV resonant Raman spectrometer which provides insight into a previously unseen world.
Deep UV resonant Raman spectroscopy has long been identified as a powerful technique as it removes the main limitations of Raman observation fluorescence. It is a proven method for measuring difficult substances, in particular organic material. When operating in the UV below 250 nm, the Raman scattering cross for simple molecules is 100 times greater than that at 785 nm, and for certain classes of organic matter, the signal can be amplified by over 10,000 times due to the resonant effect. This allows proteins to be routinely observed in a concentration of 1 mg/ml.
However, so far, the technique has been limited to academic laboratories due to the complexity and size of existing systems on the market. Often these devices use a gas pumped laser which must be cooled and purged with N2. Further working in the deep UV requires very high spectral resolution in wavelength space which puts a high degree of pressure on the spectrometer design and stability, thus systems can only be operated by experts.
“Deep UV resonant Raman has long been known to have the potential for being able to measure substances other spectroscopic techniques have not so far been capable of. Equally, the instruments currently available are extremely large, and expensive both to purchase and to maintain. This has made the technology accessible only to those organisations with physical space to house them and with significant budgets to operate them. Odin represents a departure from that as it is smaller and doesn’t have the internal mechanisms associated with high running costs.” – Dr Michael Foster, Co-Founder & Director, IS-Instruments
ODIN uses a new 229 nm laser which does not require cooling or external N2 purge combined with ISI HES spectrometer technology adapted especially for operation at this wavelength. ODIN’s size is an order of magnitude small than current instruments with a footprint that can easily fit on a desk – and yet does not sacrifice performance!
To demonstrate the power of the device, below are observations of 0.1 mg/ ml (low concentration) of tryptophan taken with ODIN compared with observations at 785 nm using one of our state-of-the-art HES instruments. The ODIN observation was made in 30 seconds and is an average of 5 frames. At 785 nm, no clear Raman spectra are observed other than that of the glass container and higher concentrations of tryptophan would simply fluoresce without producing useful spectra.